Just like other centrifuges, blood bank centrifuges also work on the principle of centrifugation. In the blood bank, it is useful in the separation of the blood components such as erythrocytes, platelets, plasma, leukocytes, etc. This process is also known as blood fractionation. It is a floor-type centrifuge with a cooling system in it. Unlike the laboratory centrifuges, it is extremely big and heavy. It may support about 4, 6, or 8 swing buckets. Each bucket holds double or triple bags. In this blog, we will be talking about the method of operation, uses, handling, and the contact details for you to purchase the blood bank centrifuge from an authorized dealer in Nepal.
Blood Bank Centrifuge Parts and Features
The three main components of the blood bank centrifuge are the rotor, motor, and compressor. The swing bucket type of rotor is supported by a brushless induction motor. The compressor is useful for cooling the centrifuge to about 4°C.
The blood bank centrifuges are controlled by the microprocessor. It can have the features of the data records, timer setup, etc. Similarly, it may have a password protection system.
It can have different features such as LCD, sensors such as rotor speed sensor, unbalance sensor, door sensor, etc. Just like the other kinds of centrifuges, the mouth of the centrifuge machine is supported by a thick gasket.
The centrifugation causes the separation of the particles. The heavier particles will settle first followed by the lighter particles. So, based on this concept, the blood bank centrifuge is used in the process of blood fractionation.
Functions of Blood Bank Centrifuge
The main purpose of the blood bank centrifuge is to separate the whole blood into different blood components such as:-
- Packed cells
- Platelet concentrate
- Platelet-rich plasma
- Buffy coat separation
Blood Bank Centrifuge Parameters Set up
According to the AABB and the DGHS manual, you need to have the following settings for better results.
RCF:- The value of the RCF should be 5000g.
Temperature Range:- For the PRP method and the Bufycoat method the temperature should range between 22°C to 24°C. Similarly, for the FFP and the Cryoprecipitate method, the temperature should be 4°C.
In the case of the timer, I suggest you set the timer value for 5 mins and check the result. Then you can alter this timer value for a better result. Since the centrifugation is affected by the geographical location, the timer value for different locations can differ slightly.
Acceleration refers to the acceleration of the rotor to obtain the set RPM/RCF from the rest position. You may set it to the maximum value. Similarly, deceleration refers to the deceleration of the rotor from the set value to the rest position. You may set this value to less than or equal to 4. If you set this value to a higher level, then the rotor will stop quickly. But this can negatively affect your result.
How to operate the blood bank centrifuge?
Follow the following steps while carrying on the blood fractionation in the blood bank.
- Turn on the machine.
- Set the parameters such as temperature, timer, RMP/RCF, acceleration, deceleration, etc, before you run the machine.
- Measure the weight of the blood bags along with the bucket on the weighing balance.
- Place the buckets into the rotor of the blood bank centrifuge. Make sure to balance the rotors. The concept of balancing the rotor is stated in the topic of a laboratory centrifuge.
- Cover the lid of the centrifuge.
- Press the start button.
(Note:- In any case, if you need to alter the temperature for centrifugation, it is better to do pre-heating or pre-cooling for about 5 mins. Else, it can affect your result. For this purpose, remove the samples from the centrifuge. Then, pre-heat or pre-cool at the required temperature. Once the centrifuge goes to the set temperature, put the sample in it. Then run the machine.
It is better to carry on similar tasks at a time. This will reduce the time for pre-heating or pre-cooling. Thus, the operation time will also be reduced).
- The machine will operate until the set timer value.
- Once the machine stops, open the lid of the blood bank centrifuge.
- Remove the blood bags.
You may carry out the following steps for handling the blood bank centrifuge.
- Keep your working place safe and clean. Else, the mouse and rats can break the wiring of the centrifuge machine.
- If possible, remove the gasket from the centrifuge and wash it with warm water. Else, wipe it with a damp cloth.
- Use Collins to clean the outer layer of the machine.
- Never try to open the lid of the machine, until it has fully stopped.
- Keep the machine in a properly ventilated room having an AC system.
Blood Bank Centrifuge in Nepal
Few suppliers deal with the trading of blood bank centrifuge in Nepal. One of them is the supplier known as the Sunrise Surgical House (+977-1-4796078/4796069/4796080/4796081), which is located in Kathmandu, Nepal. The supplier will supply this product throughout the country.
Sunrise Surgical House is a single authorized dealer of the REMI Company. The REMI company deals with blood bank centrifuges of different models. To get the brochure of different models of the blood bank centrifuges, click on the link. Based on the models the price can also differ vastly. So, to know the price for REMI, consult with the supplier.